In 1997 Barbara Danowski won 1st place in a photomicrography competition. The subject matter was moouse fibroblasts shown at 160 times. Using the fluorescence technique: Fluorescence imaging uses high intensity illumination to excite fluorescent molecules in the sample. When a molecule absorbs photons, electrons are excited to a higher energy level. As electrons ‘relax’ back to the ground-state, vibrational energy is lost and, as a result, the emission spectrum is shifted to longer wavelengths.