FALL 2020
UNION COLLEGE, SCHENECTADY, NY

Location and Meeting Time: Wold 225 MWF 10:50 AM-11:55 AM
Laboratory: WOLD 201,  WEDNESDAY 1:50-4:40 PM or THURSDAY  2:25-5:15 PM

SYLLABUS OF STUDY

COURSE OBJECTIVES

The main objectives of this course are to understand some of the fundamental concepts involved in the underlying biochemistry of a cell, and to learn how to think critically.  Emphasis will be placed on describing the functions and structure of lipids, nucleotides, and carbohydrates.  These include the role of lipids in cell membranes, the non-nucleic acid functions of nucleotides, and the role of glycosylation in protein function.  We will try and understand how structure is integrated with function, and the means by which the cell carries out these important processes.

  1. TEXTBOOKS (required)
    • Lehninger Principles of Biochemistry, Sixth Edition by David Nelson and Michael Cox (2014), published by WH Freeman (ISBN 978-1429234146). or
    • Lehninger Principles of Biochemistry, Seventh Edition by David Nelson and Michael Cox (2017), published by WH Freeman (ISBN 978-1464126116).
      • This textbook is required, as there will be assigned readings (please see lecture schedule) that will either complement or expand upon the material presented during lectures.  However, the lecture period remains the most important and efficient way of learning the material in the course.
    • The Double Helix by James Watson (1968 and 1996), published by Touchstone (Simon and Schuster) (ISBN for paperback version: 074321630X).
  2. LECTURE PRESENTATIONS
    Presentations will make use of traditional teaching tools as well as assorted electronic resources.  I will rely heavily on Nexus to transmit files including supplemental readings and slides presented in class.  If you have any problems please let me know so I can find another way to get you the required files.
  3. LABORATORY
    Students enrolled in the course will be required to attend lab as scheduled each week.  Labs will be held in the Wold Center Room 201 WEDNESDAY AND THURSDAY.  If you cannot make your scheduled lab period a particular week, you must coordinate with me to make it up at another time.  Failure to do so will result in a failing grade for the lab section of the course.
  4. GRADES
    1. Lecture-based term-tests/final exam, a final paper and in-class participation will account for 72% of your final grade as follows:
      Exam 1 12
      Exam 2 12
      Exam 3 12
      Exam 4 (during final period) 12
      BiochemTract (what’s that?) 15
      Assignments 6
      Instructor evaluation/class participation 3
    2. Lab-based grades will account for 28% of your final grade as follows:
      Lab report 10
      Mini lab reports (2) 10
      Other lab assignments 8

IMPORTANT DATES- updated 8/16/2020

Week
Wednesday
Friday
Monday
Lab
Group A Group B
1 9/9 9/11 9/14 PCR
2 9/16 9/18 9/21 Glucose binding protein isolation Purification of Glycoproteins (virtual)
Biochemtract: paper selection due Lab: PCR lab results & discussion
3 9/23 9/25 9/28 Purification of Glycoproteins (virtual) Glucose binding protein isolation
EXAM 1 NO CLASS
4 9/30 10/2 10/5 Isolation of detergent resistant membrane proteins Cholesterol Oxidase
Biochemtract: Purpose statement Lab: Glucose binding protein lab results
5 10/7 10/9 10/12 Cholesterol Oxidase Isolation of detergent resistant membrane proteins
Biochemtract: Draft Background
6 10/14 10/16 10/19 Protein Gel Electrophoresis
EXAM 2 Cholesterol oxidase intro & results
7 10/21 10/23 10/26 Protein Gel Electrophoresis
Biochemtract: Draft Approach/ Accomplishments
8 10/28 10/30 11/2 Restriction Digest Lab Analysis of Restriction Enzyme structure (virtual)
EXAM 3
9 11/4 11/6 11/9 Analysis of Restriction Enzyme structure (virtual) Restriction Digest Lab
Biochemtract: Draft Commentary Lab: Restriction enzyme results/discussion
10 11/11 11/13 11/16 Thin Layer Chromatography Lab
Lab: Restriction Enzyme structure worksheet Lab: Detergent Resistant Membrane Lab report
EXAM 4 (non-cumulative final) during Finals Period  
Final Biochemtract Due at end of Finals Period   

TENTATIVE  BIOLOGY 380 LECTURE OUTLINE  (We may cover a greater or fewer number of topics, depending upon the time available)

Week
Topics for Lectures
Readings
Sixth Edition
Seventh Edition
1 Intro
  • Chapter 1 pages 11-17, 6-11, 20-25
  • Chapter 1 pages 12-18, 7-12, 21-25
2 Water and Buffers
  • Chapter 2 pages 47-55, 58-69
  • Chapter 2 pages 47-55, 58-69
3-4 Nucleotides
  • Chapter 8 pages 281-284
  • Chapter 22 pages 910-920
  • Chapter 8 pages 287-293, 297-298, 294-297
  • Chapter 8 pages 279-282
  • Chapter 22 pages 888-898
  • Chapter 8 pages 285-292, 295-296, 292-295
5-6 Carbohydrates
  • Chapter 7 pages 243-266
  • Chapter 20 pages 823-825
  • Chapter 6 pages 220-226
  • Chapter 7 pages 266-267
  • Chapter 27 pages 1141-1142
  • Chapter 7 pages 269-276
  • Chapter 7 pages 241-265
  • Chapter 20 pages 823-825
  • Chapter 6 pages 220-225
  • Chapter 7 pages 265-266
  • Chapter 27 pages 1115-1118
  • Chapter 7 pages 267-275
7-8 Membranes & lipids
  • Chapter 10 pages 357-364, 368, 370-372
  • Chapter 21 pages 845-847
  • Chapter 12 pages 447, 450
  • Chapter 10, pages 366-370
  • Chapter 11 pages 385-389, 395-398
  • Chapter 11 pages 389-390, 394, 398-401
  • Chapter 10 pages 361-368, 372, 374-376
  • Chapter 21 pages 824-826
  • Chapter 12 pages 447, 450
  • Chapter 10, pages 370-374
  • Chapter 11 pages 387-391, 397-401
  • Chapter 11 pages 391-393, 395-397, 401-405
9-10 Synthesis & Fates of cholesterol
  • Chapter 21 pages 859-864, 870, 871-874, 864-869
  • Chapter 11 pages 413-414
  • Chapter 21 pages 874-875
  • Chapter 19 pages 763-764
  • Chapter 21 pages 837-842, 848-852, 842-847
  • Chapter 11 pages 417-418
  • Chapter 21 pages 852-854
  • Chapter 19 pages 744-745

One thought on “BIO380: Biochemistry.Fall 2020

Comments are closed.