Fun With Soil- Kelsey

Where did you obtain your soil sample?
I collected my soil from my backyard here at school. I live at 22 Union Ave. If you walk into my back yard there is a parking lot that leads to two other houses– 20r and Carriage House. I collected the soil next to the cement wall that separates our houses from a house nearby. I have attached a picture below! I dug about 2.5 inches down to get a better soil, rather than a sandy, beer can infested selection. 

Why did you choose this location?

I chose this location because the two houses behind my own tend to have a lot of outdoor parties. I thought that I would get a lot of interesting bacteria due to the wastes and circumstances this environment is exposed to.

Do you expect a lot of isolates? Why or why not?

YES! I think that I will get tons of cool stuff from this dirt. I think that because this location is exposed to both human and animal waste that I will get a lot of interesting isolates. I also think that because there was some rusty old objects mixed in with the dirt, I am even more apt to get some interesting isolates.


Have you initial observations supported this?

Yes, I have had a lot of growth on my plates! The smallest CFU that I have is 2.16×10^6. I think this is a great number. I have had a lot of diversity with my plates as well. Lots of different shapes and colors of the colonies.

What media did you choose?
I chose three types of media– LB, R2A, and AC. I found that LB had the best growths, AC had a lot of overgrowth, and R2A provided the most diverse growths (but had mycoides at the lowest dilution).


What dilutions? 

I plated 3 different dilutions on 3 medias– totaling 9 plates. In terms of the books explanation the three dilutions were 10^-1, 10^-2, and 10^-3.

Will you need to redo any? 

I did not necessarily need to redo any, but I decided to plate 1 plate of R2A with a 10^-3 dilution, 1 plate of AC with a 10^-2 dilution, and 1 plate of LB on a 10^-3 dilution. I decided to do this in order to achieve even more diversity with my plates!

How did you sample differ on the different media? 

The sample differed across media in a few ways. AC with the sample caused a major overgrowth of one colony. The R2A and sample had mycoides growth across all dilutions except for 10^-3. The LB had the most variety of growth. I think that R2A provided the most diversity, with LB a close second. I would choose them to plate more if my new plates are unsuccessful.

3 thoughts on “Fun With Soil- Kelsey

  1. I really like the location you chose to obtain your sample. I wonder if your isolates do show antibiotic activity and do turn out to be very effective drugs, what the scientists will think about this location.
    I have also run into the problem of having too many colonies grown at the 10^-3 dilution, so it was a good idea to dilute them to 10^-4 additionally. Did you notice any new or different colonies on those plates?

  2. Upon reading about where your soil sample came from, I figured we would have pretty similar results (given that similar(ish) activities occur at both of our locations). Interestingly, we both found the most diversity with the R2A plates, maybe beer decomposing bacteria grow best on R2A plates? As for the lack of diversity on the other plates, I noticed after incubating the older plates in the fridge for a few days, a lot more interesting colonies appeared. Maybe you could look on these plates and pick even more colonies?

    • Oops… I just looked at my lab notebook again, and I realized I confused my R2A and PDA plates. In fact, my R2A plates had the least diversity, so I guess our samples were less similar than I thought.

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