I tested two extracts. One extract was an isolate of mine on 10% TSA and the other extract was the same isolate on R2A. I tested the extracts on two Gram negative strains, E. carotova and E. coli. I also tested the extracts on two Gram positive strains, E. raffinosis and B. subtilis. When testing the extracts, I found no resistance observed to any of the strains. When the two isolates were tested, they had observed resistance against E. raffinosis and B. subtilis. If I were to further test the extracts, I could try different media or the same media again. I could also leave the plates out for longer to see if any antibiotic activity would show over time. However, at this point, I conclude that the isolates did not secrete any antibiotic activity into the media on which they were plated.