From my 3 (4, 6, and 11) different isolates, I obtained 5 different extracts. Isolates 6 and 11 produced two different extracts because the initial extract produced three different layers, two of which were of interest for extraction. To test my extracts for antibiotic production, I chose 2 different Gram positive and 2 different Gram negative strains to use. The Gram positive strains I chose were: S. epidermis (1) and E. raffinosis (4) and the Gram negative strains that I chose were: E. coli (2) and A. baylyi (5). I chose strains 2 and 4 because when we previously tested our isolates against the ESKAPE pathogens, I had observed inhibition of all three of my isolates against these two strains. I chose 1 and 5 because there had been slight signs of inhibition when previously tested, which may have become more prominent with a prolonged growth time.

Unfortunately when my extracts were tested against each of these strains, none of my extracts displayed antibiotic-producing activity against any of the tester strains. To further conclude that these bacterial isolates are not antibiotic producers, I could test the extracts against different strains, or test them on a different type of media. Additionally, the plates could be left to grow for a longer period of time. If the observations continued to be the same, it would be reasonable to conclude that none of my isolates are antibiotic producers.