Location of the soil sample collection: Octopus Community Garden at Union College in Schenectady, NY.                                                                                       GPS coordinates- Latitude: 42.8 degrees Longitude: -73.9 degrees

Example of 10% TSA original plate at a 10^-4 dilution (top), patch plate (middle) and streak plate of #9, 15, 20, and 23 isolates (bottom).

Gm+/Gm- Inhibition

Inhibition of both Gm+ B. subtilus (top) and Gm- E. coli (bottom) are seen for isolates 15, 20, and 23 on 10% TSA.

Gram – Control

Gram + Control

Isolate #15 Gram Stain

Isolate #20 Gram Stain

Isolate #23 Gram Stain

Gram Stain Results: Isolates 15, 20 & 23 all appear to be G+ and streptococcus, bacillus/filamentous, and coccus in shape, respectively.

Inhibition against all tester strains: Isolate #15 is inhibiting strains 1. Staphylococcus epidermidis (+)  and 2. E. coli (-), isolate #20 is inhibiting strain 6. B. subtilis (+), and isolate #23 is inhibiting strain 1. Staphylococcus epidermidis (+), all on 10% TSA.

Extraction Test Results:

Extractions were obtained for isolates #15, 20, &23. Only extract #20 showed inhibition on tester strain 1, S. epidermidis.

Results from 16S Analysis and Blast/Ribosome Database Project (RDP) Search.  I was only able to obtain results for isolate #20 (677 bp). The results show that the closest relative is Streptomyces of the vinaceus or cirratus strains with 99% identity and query coverage (Accession numbers: NR_041131.1 and NR_112388.1).