I wanted to open the category, and will post some photos soon!
Meet My Microbes – JLS
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Author Archives: salvoj
How to get your post to the right place!
So this took me a while and I had help (thanks Joey and Nicole).
1. To post, go to the menu bar at the top and click on “new” and go down to “post”.
2. Please label your post with the title of the category and your name.
3. Write your post – you can expand the menu bar (using the last icon on the right called “toolbar toggle” to provide formatting options and ways to insert images, links, etc. I hate the auto double spacing, but if you hit “shift-return” instead of just “return”, it will single space.
You might notice that I included the questions posed in my post – I just highlighted and copied these from the first post – no need to be in edit mode.
4. BE SURE to go to the menus in the right sidebar and scroll to categories. Click the proper category so the post will end up there! This also allows you to see all of yous posts when you click on your name under the “author” list on the main page.
5. I will try to initiate a post with the directions included to get things started for each assignment.
Fun with Soil – Jill
Where did you obtain your soil sample?
My soil sample came from an old cedar sawmill site in the Adirondacks. The mill was probably active in the 1920s and 30s, so while nothing remains of the mill itself, the deep pile of sawdust has settled and lacks any growth.
Why did you choose this location?
I was curious – I wouldn’t think was was a “rich” site, but might have some very selective types of microbes.
Do you expect a lot of isolates? Why or why not?
Not really. I figured the site was toxic, since after almost 100 years no trees or other plans have colonized the site.
Have you initial observations supported this?
Well – I was surprised how much grew, but I did plate the original suspension (the “no dilution” sample) as well as the 10 -1 and 10-2. But my cfu is relatively low – around 106 cfu/g.
What media did you choose? How did you sample differ on the different media?
Well – I did try all the medias to see what kind of differences I might see. I actually got more growth on the less nutrient media. Both LB, AC and R2A were very similar – low growth ( LB was 4.5 x 105) with mostly white plain looking colonies, very little diversity. PDA definitely had the most diversity with a number of dark pigmented colonies – from dark brown to tan to reddish brown. I picked more form this plate as a result. The 10% TSA had diversity, but less with pigment, some looked liked they might be filamentous. I saw no mycoides on the plates.
What dilutions? (see above)
Will you need to redo any? I did plate a 10-3 dilution of each, but I don’t expect much more. As of 24 hours, nothing has grown.
ESKAPE pathogens assignments
1. Enterococcus faecuim
2. Staphylococcus aureus
3. Klebsiella pneumoniae
4. Acinetobacter baumannii
5. Pseudomonas aeruginosa
6. Enterobacter species
2. Fun With Soil
(due Friday September 21 evening)
So I am really curious about your soil samples! so let’s share:)
Where did you obtain your soil sample? Why did you choose this location? Do you expect a lot of isolates? Why or why not? Have you initial observations supported this?
What media did you choose? What dilutions? Will you need to redo any? How did you sample differ on the different media?
2a. Share your thoughts with at least two of your classmates
(due Monday September 24 evening)
1. Introductions
For your first blog assignment, I’d like to get to know you a bit better. This (and all blog assignments) is due Friday evening.
This is pretty simple – under you name post your basic academic information (major, class) and what your future plans are – this can be long term or short term or both. Then a sentence or two about why you decided to take this course. You can be honest – if it is just to fulfill the requirement for your major, that is fine, but I will warn you I hope to convince you it will be more fun than that. Finally – a couple of things you like to do outside of your major (it be academic or non-academic). I have posted my intro to start the ball rolling:)
