The last day of my extract procedure was completed by Professor Salvo, and thus she picked the tester strains that were used. Upon inspection of the plates, no extracts were successfully isolated; none of the disks had zones of inhibition around them. This could mean that the ethyl acetate procedure wasn’t effective for these compounds, or something else could have gone wrong with the procedure. If we had more time, I would redo the extraction with methanol instead to see if the ethyl acetate was the problem.
Example plate showing no zones of inhibition.
Just curious, which testers were inhibited by your strains originally?
On the first round of ESKAPE testing, these three inhibited B. subtilis. One the second round, they no longer inhibited B. subtilis, but did inhibit Staph epi.